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osteoblast differentiation medium (odm  (Millipore)


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    Millipore osteoblast differentiation medium (odm
    Differential expression of PKA regulatory and catalytic subunits in MC3T3-E1 preosteoblasts. A, MC3T3-E1 cells were grown in the presence (+) or absence (−) <t>of</t> <t>osteoblast</t> differentiation medium <t>(ODM).</t> Transcript levels were determined using QPCR, with expression levels normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as the mean ± SD of three independent experiments. *, P < .05, **, P < .01 compared with undifferentiated cells. B, Protein lysates of MC3T3-E1 cells were analyzed by Western blotting. Actin was used as the internal control. Numbers below the panels indicate normalized (relative to actin) levels of PKA regulatory and catalytic subunits, expressed relative to the value for control shRNA, which is arbitrarily set at 1.0. This experiment was repeated at least twice with similar results, and a representative blot is shown.
    Osteoblast Differentiation Medium (Odm, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+differentiation+medium+(odm/osteoblast+differentiation+medium++odm/pmc03938543-77-5-21
    Average 90 stars, based on 1 article reviews
    osteoblast differentiation medium (odm - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Knockdown of PRKAR1A , the Gene Responsible for Carney Complex, Interferes With Differentiation in Osteoblastic Cells"

    Article Title: Knockdown of PRKAR1A , the Gene Responsible for Carney Complex, Interferes With Differentiation in Osteoblastic Cells

    Journal: Molecular Endocrinology

    doi: 10.1210/me.2013-1152

    Differential expression of PKA regulatory and catalytic subunits in MC3T3-E1 preosteoblasts. A, MC3T3-E1 cells were grown in the presence (+) or absence (−) of osteoblast differentiation medium (ODM). Transcript levels were determined using QPCR, with expression levels normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as the mean ± SD of three independent experiments. *, P < .05, **, P < .01 compared with undifferentiated cells. B, Protein lysates of MC3T3-E1 cells were analyzed by Western blotting. Actin was used as the internal control. Numbers below the panels indicate normalized (relative to actin) levels of PKA regulatory and catalytic subunits, expressed relative to the value for control shRNA, which is arbitrarily set at 1.0. This experiment was repeated at least twice with similar results, and a representative blot is shown.
    Figure Legend Snippet: Differential expression of PKA regulatory and catalytic subunits in MC3T3-E1 preosteoblasts. A, MC3T3-E1 cells were grown in the presence (+) or absence (−) of osteoblast differentiation medium (ODM). Transcript levels were determined using QPCR, with expression levels normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as the mean ± SD of three independent experiments. *, P < .05, **, P < .01 compared with undifferentiated cells. B, Protein lysates of MC3T3-E1 cells were analyzed by Western blotting. Actin was used as the internal control. Numbers below the panels indicate normalized (relative to actin) levels of PKA regulatory and catalytic subunits, expressed relative to the value for control shRNA, which is arbitrarily set at 1.0. This experiment was repeated at least twice with similar results, and a representative blot is shown.

    Techniques Used: Expressing, Western Blot, shRNA

    Related Articles

    Expressing:

    Article Title: Knockdown of PRKAR1A , the Gene Responsible for Carney Complex, Interferes With Differentiation in Osteoblastic Cells
    Article Snippet: Osteogenic differentiation was induced using osteoblast differentiation medium (ODM) composed of MEM-α with 50 mg/L ascorbic acid and 10 mM β-glycerophosphate (Sigma-Aldrich).

    Western Blot:

    Article Title: Knockdown of PRKAR1A , the Gene Responsible for Carney Complex, Interferes With Differentiation in Osteoblastic Cells
    Article Snippet: Osteogenic differentiation was induced using osteoblast differentiation medium (ODM) composed of MEM-α with 50 mg/L ascorbic acid and 10 mM β-glycerophosphate (Sigma-Aldrich).

    shRNA:

    Article Title: Knockdown of PRKAR1A , the Gene Responsible for Carney Complex, Interferes With Differentiation in Osteoblastic Cells
    Article Snippet: Osteogenic differentiation was induced using osteoblast differentiation medium (ODM) composed of MEM-α with 50 mg/L ascorbic acid and 10 mM β-glycerophosphate (Sigma-Aldrich).



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    Millipore osteoblast differentiation medium (odm
    Differential expression of PKA regulatory and catalytic subunits in MC3T3-E1 preosteoblasts. A, MC3T3-E1 cells were grown in the presence (+) or absence (−) <t>of</t> <t>osteoblast</t> differentiation medium <t>(ODM).</t> Transcript levels were determined using QPCR, with expression levels normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as the mean ± SD of three independent experiments. *, P < .05, **, P < .01 compared with undifferentiated cells. B, Protein lysates of MC3T3-E1 cells were analyzed by Western blotting. Actin was used as the internal control. Numbers below the panels indicate normalized (relative to actin) levels of PKA regulatory and catalytic subunits, expressed relative to the value for control shRNA, which is arbitrarily set at 1.0. This experiment was repeated at least twice with similar results, and a representative blot is shown.
    Osteoblast Differentiation Medium (Odm, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+differentiation+medium+(odm/osteoblast+differentiation+medium++odm/pmc03938543-77-5-21
    Average 90 stars, based on 1 article reviews
    osteoblast differentiation medium (odm - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Differential expression of PKA regulatory and catalytic subunits in MC3T3-E1 preosteoblasts. A, MC3T3-E1 cells were grown in the presence (+) or absence (−) of osteoblast differentiation medium (ODM). Transcript levels were determined using QPCR, with expression levels normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as the mean ± SD of three independent experiments. *, P < .05, **, P < .01 compared with undifferentiated cells. B, Protein lysates of MC3T3-E1 cells were analyzed by Western blotting. Actin was used as the internal control. Numbers below the panels indicate normalized (relative to actin) levels of PKA regulatory and catalytic subunits, expressed relative to the value for control shRNA, which is arbitrarily set at 1.0. This experiment was repeated at least twice with similar results, and a representative blot is shown.

    Journal: Molecular Endocrinology

    Article Title: Knockdown of PRKAR1A , the Gene Responsible for Carney Complex, Interferes With Differentiation in Osteoblastic Cells

    doi: 10.1210/me.2013-1152

    Figure Lengend Snippet: Differential expression of PKA regulatory and catalytic subunits in MC3T3-E1 preosteoblasts. A, MC3T3-E1 cells were grown in the presence (+) or absence (−) of osteoblast differentiation medium (ODM). Transcript levels were determined using QPCR, with expression levels normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as the mean ± SD of three independent experiments. *, P < .05, **, P < .01 compared with undifferentiated cells. B, Protein lysates of MC3T3-E1 cells were analyzed by Western blotting. Actin was used as the internal control. Numbers below the panels indicate normalized (relative to actin) levels of PKA regulatory and catalytic subunits, expressed relative to the value for control shRNA, which is arbitrarily set at 1.0. This experiment was repeated at least twice with similar results, and a representative blot is shown.

    Article Snippet: Osteogenic differentiation was induced using osteoblast differentiation medium (ODM) composed of MEM-α with 50 mg/L ascorbic acid and 10 mM β-glycerophosphate (Sigma-Aldrich).

    Techniques: Expressing, Western Blot, shRNA